Title: Prelab and Micropipetting excersise Overview: This lab utilizes the Amgen Bruce Wallace biotechnology lab book to teach micropipetting techniques that will be utilized in the upcoming lab that shows artificial mutation in bacteria Opening question (10 min) Explanation of Micropipette (10 min) Completion of micropipette exercises 1 and 2 on pages 1.2-1.4 of the manual (40 min) Explanation of next lab (30 min) Standards (Links): **https://riscienceteachers.wikispaces.com/LS3+p2** Objectives: Students will be able to... -effectively use a Micropipette -apply the techniques used in future labs Materials / Preparation Notes: -lab manual from Amgen (http://www.csun.edu/~jcm68622/biology/subjects/biotechnology/student/Student%20Guide%203rd%20Ed.pdf ) -P-20 micropipettes -3 solutions -Distilled H20 -1.5 ml microfuge tubes -disposable pipette tips -Permanent marker -Plastic Microfuge Rack Instruction Opening: to begin I will explain what the Micropipette is. I will show how to use it and be sure to mention how to handle it. Then I will pass out the labs taken from the Amgen lab book. I will then pass out Micropipettes and the other supplies Activities: The students will carry out the processes listen on pages 1.2-1.4 in the Amgen lab manual (http://www.csun.edu/~jcm68622/biology/subjects/biotechnology/student/Student%20Guide%203rd%20Ed.pdf ) this lab gives the students practice transferring liquid in small increments using the micropipettes. This will be useful in future labs.
Closing: To finish the assignment I will outline the lab that we will be doing in two days using the micropipettes I will also hand out the lab itself so the students can look over the lab and become accustomed to the techniques used.
Assessment Notes: There will be no formal assessment for this lesson.
Overview: This lab utilizes the Amgen Bruce Wallace biotechnology lab book to teach micropipetting techniques that will be utilized in the upcoming lab that shows artificial mutation in bacteria
Opening question (10 min)
Explanation of Micropipette (10 min)
Completion of micropipette exercises 1 and 2 on pages 1.2-1.4 of the manual (40 min)
Explanation of next lab (30 min)
Standards (Links):
**https://riscienceteachers.wikispaces.com/LS3+p2**
Objectives:
Students will be able to...
-effectively use a Micropipette
-apply the techniques used in future labs
Materials / Preparation Notes:
-lab manual from Amgen (http://www.csun.edu/~jcm68622/biology/subjects/biotechnology/student/Student%20Guide%203rd%20Ed.pdf )
-P-20 micropipettes
-3 solutions
-Distilled H20
-1.5 ml microfuge tubes
-disposable pipette tips
-Permanent marker
-Plastic Microfuge Rack
Instruction
Opening: to begin I will explain what the Micropipette is. I will show how to use it and be sure to mention how to handle it. Then I will pass out the labs taken from the Amgen lab book. I will then pass out Micropipettes and the other supplies
Activities: The students will carry out the processes listen on pages 1.2-1.4 in the Amgen lab manual (http://www.csun.edu/~jcm68622/biology/subjects/biotechnology/student/Student%20Guide%203rd%20Ed.pdf ) this lab gives the students practice transferring liquid in small increments using the micropipettes. This will be useful in future labs.
Closing: To finish the assignment I will outline the lab that we will be doing in two days using the micropipettes I will also hand out the lab itself so the students can look over the lab and become accustomed to the techniques used.
Assessment Notes: There will be no formal assessment for this lesson.